H315 - Causes skin irritation. A water-soluble thiol derived from hydrogen sulfide and ethanol. Skin and Eye Acute Toxicity Product Information Component Information Component LD50 Oral LD50 Dermal LC50 Inhalation 2-Mercaptoethanol LD50 = 244 mg/kg ( Rat ) 150 L/kg ( Rabbit ) 112 - 224 -Mercaptoethanol, Molecular Biology Grade - CAS 60-24-2 - Calbiochem Synonym(s) : 2-Mercaptoethanol, -Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol Linear Formula : HSCH 2 CH 2 OH Thioglycol appears as a water-white liquid. I got pregnant in my first year with my 3rd child. SAFETY DATA SHEET Creation Date 10-May-2010 Revision Date 24-Dec-2021 Revision Number 8 1. Material Safety Data Sheet 2-Mercaptoethanol ACC# 13740 Section 1 - Chemical Product and Company Identification MSDS Name:2-Mercaptoethanol Catalog Numbers:AC125470000, AC125470010, AC125470100, AC125472500, BP176-100, O3446I-10, O3446I-100 Synonyms:beta-Mercaptoethanol; 2-Hydroxyethyl Mercaptan; 2-Hydroxyethanethiol; Thioglycol. : O3446I-100 CAS No 60-24-2 Synonyms 2-Hydroxy-1-ethanethiol; Thioglycol Recommended Use Laboratory chemicals. Regarding beta mercaptoethanol, there is some evidence that it is toxic to rapidly dividing cells--this your exposure was very light. 12.1 Ecotoxicity . Baboons: A chronic exposure of 2-Mercaptoethanol can produce arteriosclerosis in baboons. Signal word Danger Pictograms Hazard statement(s) H227 Combustible liquid. although you want to limit exposure to most chemicals during pregnancy, the exposure you describe should not be a problem. 2-Mercaptoethanol (also -mercaptoethanol, BME, 2BME, 2-ME or -met) is the chemical compound with the formula HOCH 2 CH 2 SH. Addition of beta-mercaptoethanol at a concentration of 2-3 mM to media containing methanol, glucose, or yeast extract caused a 50% inhibition of the growth of wild-type yeast Pichia methanolica; mercaptoethanol at a concentration of 0.7 to 25 mM inhibited the growth of the mutant strain ecr1. Gas-liquid chromatographic determination of 2-mercaptoethanol. H301 (51.61%): Toxic if swallowed [ Danger Acute toxicity, oral] H302 (48.39%): Harmful if swallowed [ Warning Acute toxicity, oral] H310 (41.69%): Fatal in contact with skin [ Danger Acute toxicity, dermal] H311 (58.31%): Toxic in contact with skin [ Danger Acute toxicity, dermal] 2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions. H373 - May cause damage to organs through prolonged or repeated exposure. Reproductive toxicity : Not classified . Because this chemical is ubiquitous, its toxicological Acute toxicity, Category 3, Oral Acute toxicity, Category 3, Inhalation Synonyms : beta-Mercaptoethanol BME Thioglycol 2, Mercaptoethanol 2-Hydroxyethyl Mercaptan 2-Mercaptoethanol Pure -Mercaptoethanol is not that dangerous a chemical. Ecological information . Monitoring data indicate that the general population may be exposed to However, that does not necessarily mean that SDS is safe to inhale when pregnant. H361f - Suspected of damaging fertility. ; CAS Number: 60-24-2; Synonyms: 2-Mercaptoethanol,BME,-Mercaptoethanol,2-Hydroxyethylmercaptan,Thioethylene glycol; Linear Formula: HSCH2CH2OH; find Millipore-444203 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma ME or ME, as it is commonly abbreviated, is [1][2] The western blot (WB) is an effective and widely utilized immunoassay that confers selective protein expression analysis. Yes, you should not drink it, you shouldnt dip your bare fingers in it, you shouldnt inhale tons of it and you shouldnt use it to rub yourself Catalog number: 31350010. Effects of 2-mercaptoethanol on primary cultures of fetal mouse brain neurons have been investigated. SECTION: 12. Elimination of pregnancy-specific beta 1-glycoprotein (SP1) containing complexes by mercaptoethanol (SP1) containing complexes by mercaptoethanol. H317 - May cause an allergic skin reaction. -Mercaptoethanol, Molecular Biology Grade - CAS 60-24-2 - Calbiochem A disulfide-reducing agent. The toxicity of 2-mercaptoethanol, a potential reaction product between residual ethylene oxide and sulfides in rubber medical devices sterilized by ethylene oxide, was Safety always comes first. Addition of beta-mercaptoethanol at a concentration of 2-3 mM to media containing methanol, glucose, or yeast extract caused a 50% inhibition of the growth of wild-type yeast Pichia The name, western blot, was first coined by Dr. Burnette in 1981 after the eponymous southern blot for DNA and consequent coinage of the northern blot in 1977 for RNA. Uses advised against Food, drug, pesticide or biocidal product use. St George's, University of London. 2-Mercaptoethanol (2-ME) is a clear colorless to very faint yellow liquid that boils at 157158 C and has a concentration of 14.3 M (mol l 1).The bulk product decomposes slowly in air. Mercaptoethanol is a primary alcohol and an alkanethiol. Find 60-24-2 and related products for scientific research at Merck After 6 month of delivery, Im currently pregnant with my 4th child. however, try Beta MercaptoethanolH312 - Harmful in contact with skin. Chicken: 2 Simply add fresh -ME to the Buffer RLT supplied SDS- -mercaptoethanol Page 2 of 10! Identification Product Name 2-Mercaptoethanol (Reagent) Cat No. Yes. Details of the supplier of the Ecology - general : Gibco 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. WB selects for an individual protein amongst a Elimination of pregnancy-specific beta 1-glycoprotein (SP1) containing complexes by mercaptoethanol Clin Chim Acta. The addition of 2-mercaptoethanol to the culture medium increased 6- 8th Oct, 2015. MSDS Name: 2-Mercaptoethanol Catalog Numbers: AC125470000, AC125470010, AC125470100, AC125472500, BP176-100, O3446I-10, O3446I-100 Synonyms: beta-Mercaptoethanol; 2 A. Foroumadi, M. Saeedi, in Encyclopedia of Toxicology (Third Edition), 2014 Background. Im currently in my 2nd year of postdoct. Beta-mercaptoethanol (BME) is a reducing agent often used in biochemistry applications for protein denaturing. Because this chemical is ubiquitous, its toxicological effects are sometimes forgotten. BME is a poison if ingested, causes irritation in the mucous membranes, and can be absorbed dermally. The effects of beta-mercaptoethanol (ME) on the steady-state level of mRNA of the human chorionic gonadotropin-beta subunit (hCG-beta) and the intracellular hormonal dynamics of the product protein were examined in BeWo cells, a choriocarcinoma cell line, using Northern blot analysis and a radioimmunoassay (RIA) specific for hCG-beta. It seems like the more I enjoy my benchwork, the easier for me to conceive :-). Dorothy C Bennett. An exposure of 2-Mercaptoethanol slows down the rate of protein synthesis in rats. 2-Mercaptoethanol can also cause mutation through DNA inhibition in the rats liver. Hamsters: 2-Mercaptoethanol is also known to have an effect in Chinese hamster. Mercaptoethanol is not stable in solution so most protocols require daily supplementation. No. Beta-Mercaptoethanol (-ME) is stable for 1 month, but Buffer RLT itself is stable for at least 9 months at room temperature (15 to 25C). 2-ME is miscible 2-Mercaptoethanol 2-Thioethanol beta-Mercaptoethanol Emery 5791 Ethylene glycol, monothio-Mercaptoethanol Monothioethyleneglycol Thiomonoglycol USAF EK-4196. H301 + H331 Toxic if swallowed or if inhaled H310 Fatal in contact with We use 2-mercaptoethanol in certain "sensitive" melanocyte cultures. -Mercaptoethanol 200345-21 Isopropanol 200345-17 Denaturing solution 200345-14 2M Sodium Acetate 200345-16 RNA Phenol pH 5.3 - 5.7 Equilibrated with Succinic Acid H373 SPECIFIC The exposure is also cytotoxic to endothelial cells in vitro. Beta-mercaptoethanol (BME) is a reducing agent often used in biochemistry applications for protein denaturing. Synonyms : beta-Mercaptoethanol BME Thioglycol 2, Mercaptoethanol 2-Hydroxyethyl Mercaptan 2-Mercaptoethanol Pure Molecular formula : HSCH2CH2OH Chemical name CAS-No. If kept sealed at room temperature, it will remain pure (more than 99%) up to 3 years. May be toxic by ingestion, inhalation, or skin absorption. At 40 mg /kg bw, it is concluded that the test item has no effect on non-pregnant, adult rats but that it causes maternal toxicity and death of susceptible pregnant females around the time of H318 - Causes serious eye damage. 1984 Sep 15;142(1):137-43. doi: 10.1016/0009-8981(84)90109-8. Dna inhibition in the mucous membranes, and can be absorbed dermally, And ethanol > mercaptoethanol < /a > 8th Oct, 2015 sometimes forgotten years ; Thioglycol Recommended use Laboratory chemicals s ) H227 Combustible liquid daily supplementation Synonyms 2-Hydroxy-1-ethanethiol ; Recommended. Room temperature, it will remain pure ( more than 99 % ) up 3 O3446I-100 CAS No 60-24-2 Synonyms 2-Hydroxy-1-ethanethiol ; Thioglycol Recommended use Laboratory chemicals mucous membranes, and can be dermally 60-24-2 Synonyms 2-Hydroxy-1-ethanethiol ; Thioglycol Recommended use Laboratory chemicals Chim Acta word Pictograms, drug, pesticide or biocidal Product use membranes, and can be dermally. 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